Method development and validation of RP-HPLC method determination of vericiguat in bulk and formulation

Abstract: Analytical Chemistry is a measurement of science consisting of a set of powerful ideas and methods that are useful in all fields of science and medicine Attempts were made to develop RP-HPLC method for simultaneous estimation of Vericiguat from Tablet. For the RP - Agilent Tech. Gradient System with Auto injector, DAD Detector Reverse Phase (Agilent) C18 column (4.6mm x 100 mm;2.5µm), a 20µl injection loop and UV730D Absorbance detector and running chemstation 10.1 software.
Methanol: water (0.1% acetic acid), (50:50) v/v, pH 3.was used as the mobile phase for the method. The detection wavelength was 258nm and flow rate was 0.8 ml/min. In the developed method, the retention time of Vericiguat was found to be being 5.268min. The method has been found to be better than previously reported method, because of its less retention time, isocratic mode and use of an economical and readily available mobile phase, readily available column, UV detection and better resolution of peaks.