Molecular identification of bacteria in ready-to-eat cow intestines from Ado-Ekiti Abattoir, Nigeria, Using 16S rRNA Sequencing

Background: Abattoir meat, particularly ready-to-eat cow intestine, is a valuable source of nutrients but may also harbor pathogenic microbes that compromise food safety. The present study investigated bacterial contaminants of freshly slaughtered cow intestine obtained from an abattoir in Ado-Ekiti, Nigeria, using 16S rRNA sequencing. Methods: Samples of freshly slaughtered cow intestines were aseptically selected and subjected to bacteria isolation. DNA was extracted, amplified by PCR for 16S rRNA gene, and sequenced. Results: Various bacterial species were found, including Providencia vermicola (97.74% and 95.98% similarity), Pseudomonas pseudoalcaligenes (99.93%), Comamonas testosterone (84.71%), Escherichia coli (99.32%), Pseudomonas mendocina (99.77%), Bacillus subtilis (99.78%), and Comamonas kerstersii (99.77%). The presence of opportunistic and pathogenic bacteria is of public health significance for meat processing hygiene. Conclusion: Freshly slaughtered cow intestines in Ado-Ekiti abattoir are harboring diverse populations of bacteria, including potential pathogens. Improved abattoir hygiene, strict regulatory enforcement, and consumer education are paramount to reducing foodborne risks.