Protective effects of naringin in indomethacin-induced gastric ulcer in rats

This study aimed to investigate the potential protective effects of Naringin (NA) in Indomethacin (IND)-induced gastric ulcers in rats. Forty adult male Sprague Dawley rats were randomly divided into four groups; the control group was given intragastric (ig) saline for 10 days, the IND group was given ig saline for 10 days and given a single dose of IND (100 mg/kg, ig) on the 11th day, and the NA+IND and RAN+IND groups were administered NA (100 mg/kg, ig) and RAN (5mg/kg, ig) for 10 days, respectively. These final two groups were administered single dose IND (100 mg/kg, ig) on the 11th day, and 24 hours after IND administration, the rats were decapitated under anesthesia. The stomach tissues were examined to calculate the ulcer index and analyze inflammation and apoptosis markers. In addition, microscopic examination of the stomach tissues was performed. The results showed that IND induced gastric ulcers and increased tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP), inducible nitric oxide synthase (iNOS), and caspase-3 levels while decreasing cyclooxygenase-2 (COX-2). NA exhibited a protective effect by preventing these parameter changes. In addition, NA corrected the histopathological abnormalities caused by IND in stomach tissue. These results indicate that NA might protect against the effects of IND in rats.