Phytoremideation of ethylene glycol induced renal calculi in the vertebrate model Rattus norvigicus

Multicellular organisms have special feature to concentrate and excrete nitrogenous wastes from the body. Normally kidneys in vertebrates have extensive role in the filtration and elimination function. Urinary calculi or stones are the most common cause for chronic / acute obstruction of urinary system. Pathologically urolithiasis (nephrolithiasis) relates with kidney stone disease. Understanding of the pathophysiology of nephrocytes with altered blood and urine constituents found to be thrust area of urolithiasis; therefore, present investigation has intended to focus on mechanism for development of calculus in kidney and phytoremideation against Ethylene glycol (EG) induced nephritic cells. With the intension vertebrate model rat have been used to understand pathogenesis and dose dependent recovery mechanism of two plant extract of Tribulus terrestris (Tt) and Amranthus cruentus (Ac). All animals were subjected to EG induction as a first part of experimental design.  The recovery mechanism considering urine profile and histological observations were carried out with treatment of plant extracts. Observations in relations to doses compensation against EG were statically documented.  Results of the different toxic concentration and exposure period were observed histologically and interpreted for comparative effects of plant extract (AC: TT) pertaining depleted percentage of crystal formation responsible for stone development. The overall mechanisms of phytoremideation were critically studied and interpreted in universally accepted   vertebrate experimental model Rattus norvigicus.