Histopathological liver of one day old and two-week-old chicks exposed to carbofuran during embryonic period

Objective: This study aims to determine the effect of carbofuran exposure during the embryonic period on the histopathological images of the liver of one day old and two-week-old chicks after they hatch.

Method: 60 embryonated chicken eggs from broiler parent stock with an average weight of 62.064 mg were divided into two observation groups: one day old chicks and two-week-old chicks after they hatched. Carbofuran was dissolved in aquabidest and then was injected into the egg yolk at a dose of 0.0106 mg/0.1 ml/egg (P1) and 0.0127 mg/0.1 ml/egg (P2). The control group (P0) was injected with 0.1 ml of aquabidest. All eggs were incubated in an electric incubator until they hatched. The livers of one day and two-week-old chicks were collected for HE staining. The variables observed were the level of liver cell necrosis in the central vein and Kiernan's triangle. Data were analyzed using Kruskal Wallis and it continued with multiple comparison tests.

Results: The results showed that the carbofuran group of one day old chicks treated with at a dose of 0.0106 mg/0.1 ml/egg had a significant effect on necrosis of liver cells in the central vein and in Kiernan's triangle. Moreover,the carbofuran group of two week old chicks treated at a dose of 0.0127 mg/0.1 ml/egg (P2) and 0.0106 mg/0.1 ml/egg (P1) had a significant effect compared to the P0 group (p<0.05).

Conclusion Carbofuran insecticide at a dose of 0.0127 mg/0.1 ml/egg was proven to cause the necrosis of liver cells in the central vein and in Kiernan triangle of one day and two-week-old chicks when they were exposed to carbofuran during the embryonic period.