Verification of the analytical performance of the serum haptoglobin assay on the Abbott Alinity ci

Loubna Yacoubi; Mouad Harandou; Amina Himri; Zainab Kajeiou; Sabah Mokhtari; Issam Mokhtari; Nisma Douzi; El houcine Sebbar; Mohammed Choukri

doi:10.30574/gscbps.2024.26.1.0024

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Verification of the analytical performance of the serum haptoglobin assay on the Abbott Alinity ci

Loubna Yacoubi ^{1, 2, *}, Mouad Harandou ^{1, 2}, Amina Himri ^{1, 2}, Zainab Kajeiou ^{1, 2}, Sabah Mokhtari ^{1, 2}, Issam Mokhtari ^{1, 2}, Nisma Douzi ^{1, 2}, El houcine Sebbar ^{1, 2} and Mohammed Choukri ^{1, 2}

1 Central Laboratory, Mohammed VI University Hospital, Oujda, Morocco.

2 Mohammed First University, Faculty of Medicine and Pharmacy of Oujda, Morocco.

Research Article

GSC Biological and Pharmaceutical Sciences, 2024, 26(01), 242–246.

Article DOI: 10.30574/gscbps.2024.26.1.0024

DOI url: https://doi.org/10.30574/gscbps.2024.26.1.0024

Publication history

Received on 07 December 2023; revised on 15 January 2024; accepted on 18 January 2024

Abstract

Introduction: The aim of our study is to evaluate the haptoglobin assay on the Alinity ci automated system. This evaluation is part of an overall approach to verifying the methods used in the central laboratory of the Mohammed VI University Hospital of Oujda , with a view to compiling an accreditation file in accordance with the requirements of standard NF ISO 15189.

Materials and methods: The working methodology adapted by our study is based on the recommendations of the protocol of the COFRAC accreditation technical guide SH GTA 04.

Verification involved assessing the repeatability and reproducibility of Alinity ci .

Results: The results obtained for the various haptoglobin assay verification criteria show satisfactory repeatability for all three levels, with CV1=1.10%, CV2=0.78% and CV3=1.07% respectively. intra-laboratory reproducibility was satisfactory for all three levels, with CV1=1.79%, CV2= 4.2% and CV3=1.62% respectively.

Discussion: Verification of an analytical method is an essential step in guaranteeing that the result obtained is as close as possible to the reference value of a sample. Comparing our results with the CV adopted by the SFBC, we can see that the results are in line with and below the tolerated limits.

Conclusion: We can therefore conclude that the Abbott Alinity ci system meets the requirements set by scientific societies for the determination of haptoglobin.

Keywords

Haptoglobin; Verification; Repeatability; Reproducibility; Alinity ci

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Loubna Yacoubi, Mouad Harandou, Amina Himri, Zainab Kajeiou, Sabah Mokhtari, Issam Mokhtari, Nisma Douzi, El houcine Sebbar and Mohammed Choukri. Verification of the analytical performance of the serum haptoglobin assay on the Abbott Alinity ci. GSC Biological and Pharmaceutical Sciences, 2024, 26(1), 242-246. Article DOI: https://doi.org/10.30574/gscbps.2024.26.1.0024

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Verification of the analytical performance of the serum haptoglobin assay on the Abbott Alinity ci

Loubna Yacoubi ^{1, 2, *}, Mouad Harandou ^{1, 2}, Amina Himri ^{1, 2}, Zainab Kajeiou ^{1, 2}, Sabah Mokhtari ^{1, 2}, Issam Mokhtari ^{1, 2}, Nisma Douzi ^{1, 2}, El houcine Sebbar ^{1, 2} and Mohammed Choukri ^{1, 2}

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Verification of the analytical performance of the serum haptoglobin assay on the Abbott Alinity ci

Loubna Yacoubi 1, 2, *, Mouad Harandou 1, 2, Amina Himri 1, 2, Zainab Kajeiou 1, 2, Sabah Mokhtari 1, 2, Issam Mokhtari 1, 2, Nisma Douzi 1, 2, El houcine Sebbar 1, 2 and Mohammed Choukri 1, 2

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Loubna Yacoubi ^{1, 2, *}, Mouad Harandou ^{1, 2}, Amina Himri ^{1, 2}, Zainab Kajeiou ^{1, 2}, Sabah Mokhtari ^{1, 2}, Issam Mokhtari ^{1, 2}, Nisma Douzi ^{1, 2}, El houcine Sebbar ^{1, 2} and Mohammed Choukri ^{1, 2}