Specific and non-specific parameters determination and chemical content tests of the combination of ethanol extracts of Acalypha indica Linn and Peperomia pellucida (L) H.B.K.

Indonesian people have long used herbal plants as medicine. Herbal plants used by Indonesian people include cat root herb (Acalypha indica Linn) and suruhan herb (Peperomia pellucida (L) H.B.K). Previous research showed that combining cat root herb extract and surah herb was safe for rat kidneys. This research aims to test specific and non-specific standard parameters in the combination of the two extracts so that they are safe and have good quality that meets the requirements and to know the chemical content of the extracts combination. The specific parameters used in this research were dissolved compound levels (water and ethanol soluble compound levels) and non-specific parameters, namely drying loss, water content, and ash content. The water-soluble compound content obtained was 7.44%, and the ethanol-soluble compound content was 4.9%. The drying loss was 3.29%, extract water content was 4.41%, total ash content was 7.39%, and acid-insoluble ash content was 4.37%. Based on the results of this research, it can be concluded that several specific and non-specific parameters meet the established quality standards. The identification of the extracts combination shows the presence of alkaloids, flavonoids, tannins, saponins, glycosides and cyanogenic glycosides, but does not contain anthraquinone glycosides, and the total alkaloid content is 0.31%.