Effect of organophosphate pesticide (chlorpyrifos, fipronil, and malathion) on certain organs of Rattus rattus

The study was conducted to evaluate efficacy of pesticide that effect on histopathology of Rattus rattus under laboratory conditions of 30±20 ˚C and 60±50% room humidity in the Biocontrol laboratory at the Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad. Baits were prepared by pesticides chlorpyrifos, fipronil and malathion as well as adding flour, sugar, cheese, and oil.  Baits were given to rats as 4000mg/kg of body weight. Animals were kept into four groups, one as control while others as treated with Pesticides chlorpyrifos, fipronil, and malathion. Observations concerning the morphological changes were recorded in the laboratory on regular basis. Visible physiological changes observed. Highly significant (at p ≤0.01) variations found in the results of physical parameters like feed consumption and water intake. Histopathological records showed momentous changes in tissues of the kidney and liver. Necrosis of tissues was noteworthy in all organs. ANOVA was applied on the histopathological level that showed significant variations due to the application of the pesticide.