Some biological potential of silver nanoparticles synthesized from Ocimum basilicum L.

In our study, the ability to produce silver nanoparticles (AgNP) by green synthesis method using Ocimum basilicum L. plant was investigated. The color of the extract changed from yellow to brown, proving the presence of AgNPs. Scanning electron microscopy (SEM) and energy dispersed spectra (EDS) were used to characterized AgNPs. AgNPs ranged from 15 nm to 22 nm in size and were mostly spherical. The antimicrobial potential of AgNPs was tested against four bacterial and one yeast cultures by disc diffusion and minimum inhibitory concentration (MIC) methods. AgNPs significantly inhibited all test cultures. The highest antimicrobial and antibiofilm activities of plant extract and AgNP were found against Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231, respectively. In free radical scavenging activity (DPPH), the higher antioxidant activity were found 56.31±0.73% (plant extract) and 30.71±1.35% (AgNP). The higher 2,2'- Azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS•+) cation radical scavenging acitivty were also determined 55.87±1.38% and 39.56±1%, respectively.