Isolation, identification and antibiogram of bacterial flora from rectum of horses

In the present study, we isolated, identified and characterized bacteria in rectal swabs from apparently healthy horses around the campus of the Bangladesh Agricultural University (BAU). The study also focused on the determining the prevalence and investigation of drug sensitivity and resistant pattern of the isolates. For this purpose, 20 rectal swab samples were collected from Veterinary Teaching Hospital, BAU; D. C. park (Joinul Abedin Park), Mymensingh and Torar mor, Mymensingh. Most of these samples were found positive for E. coli, Staphylococcus spp. and Salmonella spp. Based on the origin of the samples, the highest prevalence of E. coli, Staphylococcus aureus and Salmonella spp. was found in BAU campus and Torar mor (100%), BAU campus and Torar mor (100%) and Joinul Abedin Park (80%) respectively. The overall prevalence of E. coli, S. Aureus and Salmonella spp. was 93%, 86.67% and 24.44% respectively. For the confirmation of identification and characterization of these bacteria staining, cultural, biochemical studies and Polymerase Chain Reaction (PCR) were accomplished. The antibiogram profiles exhibited that E. coli, S. Aureus and Salmonella spp. were highly sensitive to Erythromycin, Gentamycin and Ciprofloxacin respectively