Probiotics in dermatological therapy and skincare

Probiotics are characterised as “live microorganisms'' and use of probiotics has shown promising effects on the prevention and treatment of different inflammatory skin disorders such as acne and atopic dermatitis. The Lactobacillus sp. were isolated using fermented rice water. Fermented rice water was serially diluted and plated on MRS Medium plates in which the bacterial isolates were observed to be round, flat, slightly mucoid, creamy in colour. The Lactobacilli was confirmed by Gram staining and the slide was observed to be gram positive rod shaped. Biochemical test was also performed in which TSI slant was observed with red slant, yellow butt without gas and H2S formation. Screening was done for Lactobacillus sp. for probiotic properties. Optimal growth was determined at 560 nm at pH 5.5. NaCl concentration tolerance at 4% was observed to be optimal. The antimicrobial activity of the isolated Lactobacillus was identified by a well diffusion method and was diameter up to 0.8, 0.6 & 1.2 cm. Supernatants of each sample of Lactobacillus were monitored for antibacterial activity against indicator bacteria Bacillus, Pseudomonas inoculated on Nutrient agar and presence of clear zones of inhibition around the wells were observed. Antibiotics sensitivity test Disk diffusion method was performed and zones of inhibition around the disks were observed. Shea butter, raw honey and aloe vera gel along with that Pellets of Lactobacillus species were added and tea tree oil and essential oils were added drop by drop and this probiotic face serum was stored. The lab made face serum was further dissolved insterilised distilled water and a loopful was inoculated to the Nutrient medium containing petri plates for determining the viability of lactobacillus species after incubation.