1 Department of Pharmacology, Sciences Faculty, University of Antananarivo, Antananarivo, Madagascar.
2 Department of Agronomy and Agri-Food, Engineering School of Agronomy and Environment, High Institute of Technology of Ambositra, Madagascar.
3 Department of Organic Chemistry, University of Antananarivo, Antananarivo, Madagascar.
Received on 10 January 2026; revised on 26 February 2026; accepted on 28 February 2026
The Apocynaceae family is known for its richness in cardiotonic glycosides. This study aimed to investigate the cardiotonic activity of the hydroalcoholic extract from the leafy stems of Petchia erythrocarpa on experimentally induced heart failure using isolated frog heart. Cardiac contraction force, heart rate, diastolic filling time, and cardiac output were evaluated.
The extract was perfused in the isolated heart at concentrations ranging from 0.15 to 0.9 mg/mL. Within this range of concentration, the extract significantly increased cardiac contraction force by 18 to 73 % (p < 0.05), the cardiac output from 0.51 ± 0.07 to 0.91 ± 0.05 mL/s (p < 0.05), the diastolic duration from 0.13 ± 0.003 to 0.24 ± 0.009 s (p<0.05), while it decreases the heart rate from 44.5 ± 1.1 to 31.3 ± 0.6 beats/min (p < 0.05). These results indicate that the extract possesses positive inotropic and lusotropic effects and a negative chronotropic effect, confirming its potential as a cardiotonic agent.
Cardiotonic; Frog; Isolated heart; Petchia erythrocarpa
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Delore Soaviherimbola RAZAFIMAHAZORO, Roméo RAZANADRABENAFINDRA, Kanto RATOVONARINDRA, Nathaniel QUANSAH, Maonja Finaritra Sitrakiniavo RAKOTONDRAMANGA and Patricia RANDRIANAVONY. Phytochemical analysis and cardiotonic activity of hydroalcoholic extract of Petchia erythrocarpa (Vatke) Leeuwenb. (APOCYNACEAE) in isolated frog heart. GSC Biological and Pharmaceutical Sciences, 2026, 34(3), 001-008. Article DOI: https://doi.org/10.30574/gscbps.2026.34.3.0088