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Research and review articles are invited for publication in September 2026 - Vol. 36, Issue 3 

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Isolation of IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation

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  • Isolation of IgG From Chicken Serum Using a Combination of Coconut Caprylic Triglyceride and Ammonium Sulfate Precipitation
  • Isolation of IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation

Sulaiman Ngongu Depamede *, Made Sriasih, Adji Santoso Sudradjat and Nurul Azizah

Faculty of Animal Science, University of Mataram, Mataram - NTB, Indonesia.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 27(02), 302–306.
Article DOI: 10.30574/gscbps.2024.27.2.0241
DOI url: https://doi.org/10.30574/gscbps.2024.27.2.0241
Received on 21 March 2024 revised on 25 May 2024; accepted on 28 May 2024
This study aimed to isolate IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation techniques. This study used serum from chickens that had previously been vaccinated repeatedly using a commercial rabies vaccine. Initially, the serum was treated with 2.5% coconut caprylic triglyceride, followed by serum IgG precipitation using 40% ammonium sulfate. The 10% SDS-PAGE analysis showed that a protein of about 190 kDa with a purity above 95% was successfully isolated. Whether the isolate protein is IgG and specific to rabies antigen still needs to be investigated further.
Antibody; Coconut caprylic triglyceride; Chicken serum; IgG; IgY
https://gscbps.gsconlinepress.com/sites/default/files/fulltext_pdf/GSCBPS-2024-…

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Sulaiman Ngongu Depamede, Made Sriasih, Adji Santoso Sudradjat and Nurul Azizah. Isolation of IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation. GSC Biological and Pharmaceutical Sciences, 2024, 27(2), 302-306. Article DOI: https://doi.org/10.30574/gscbps.2024.27.2.0241


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