Investigation of the effect of Polygonum cognatum Meissn. ethanol extract Bax, Caspase-3, Bcl-2, NF-B and NRF-2/HO-1 pathways in streptozotocin induced diabetic rats

In this study, it was investigated of the effect of Polygonum cognatum Meissn. ethanol extract Bax, Bcl-2, Caspase-3, NF-kB and NRF-2/HO-1 pathways in streptozotocin induced diabetic rats. A total of 24 healthy Spraque-Dawley male rats were randomly divided into four groups containing of 6 rats per group as Control; Diabetes mellitus (DM); Polygonum cognatum Meissn. ethanol extract (PCE) and DM+PCE. Experimental diabetes was induced by a single dose of 60 mg/kg/i.p Streptozotocin (STZ) injection for DM and DM+PCE groups. The animals showing diabetes (Blood glucose level >250 mg/dL) will be selected for diabetes groups in 7 th days. PCE was given at a dose of 10 mg/kg /day/p.o via gavage 20 days. All of rats were sacrificed on 20 th day, taken blood and dissected kidney tissues ender anesthesia. Bax, Bcl-2, Caspase-3, COX-2, HO-1, NF-kB and Nrf-2 expression levels were determined by western blotting in kidney tissue. Compared with the control and diabetes groups, Bax, Caspase-3, COX-2 and NF-kB expression levels increased (p<0.001); Bcl-2, HO-1 and Nrf-2 expression levels were decreased in diabetes group. PCE given with STZ decreased Bax, Caspase-3, COX-2 and NF-kB expression levels (p<0.001); Bcl-2, HO-1 and Nrf-2 expression levels were increased. PCE treats STZ-induced diabetic kidney injury by regulating apoptosis parameters such as Bax, Bcl-2, Caspase-3, and inflammation pathways such as, NF-kB, COX-2, Nrf-2 and HO-1 against STZ-induced diabetes. It was concluded that PCE can be used as a therapeutic agent after determining the molecular processes behind the therapeutic benefits of PCE against kidney damage in STZ-induced diabetes. kidney injury kidney damage. According to the biochemical findings, PCE 10 mg/kg treatment dose decreased in kidney Bax Bcl-2, Caspase-3 and NF-kB pathways, increased in kidney Nrf-2 and HO-1 protein expression levels when administered with STZ was presented as a pioneering study in the literature.