Evaluation of the hemogram of Oryctolagus cuniculus envenomus

The overall objective of this study was to evaluate the hemolysing action of Naja nigricollis venom on rabbit blood. To carry out this study, three batches of three rabbits were formed with two control batches and one experimental batch. Each control lot is composed of three rabbits (males or females) while the experimental lot is composed of two males and one female. Each rabbit from the control lots was separately collected in the purple tube (EDTA) and transported to the laboratory for analysis. The rabbits from the experimental batch were also collected distinctly a few minutes after the injection of the venom of Naja nigricollis for the analysis of haematological parameters. However, before the analysis of the hematological parameters of the rabbits from the control and experimental batches, an in vitro hemolysis test of Naja nigricollis venom was performed to verify its hemolysing power. The results showed that Naja nigricollis venom has a dose-dependent in vitro hemolysing power. As for the haemogram, it revealed that the venom of Naja nigricollis has a decreasing effect on blood cells (red and white blood cells), on haemoglobin and on haematocrit, and an elevation on MGVs thus promoting anaemia.