1 Department of Periodontology, Justus-Liebig-University, Giessen, Germany.
2 BRACE GmbH, Karlstein, Germany.
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 21(01), 188–196.
Article DOI: 10.30574/gscbps.2022.21.1.0198
Received on 17 May 2022; revised on 13 October 2022; accepted on 16 October 2022
Polyhexanide (polyhexamethylene biguanide) (PHMB) is a polymeric substance that is broadly used as disinfectant and antiseptic. It was shown to be suitable for clinical use in critically colonized or infected acute and chronic wounds since it exhibits a broad antimicrobial spectrum combined with good cell and tissue tolerability and low risk of contact sensitization. Furthermore a wound healing promoting effect was reported. The aim of this work was to develop a PHMB containing slow release device for sustainable disinfection of bacterial infected periodontal pockets and other colonized niches of the body.
A preparation of 10% PHMB in paraffin microcapsules with the size of 200 µm was developed. Its antimicrobial and releasing properties were investigated in vitro using dissolving experiments in hanks buffered salt solution (HBSS) with and without fetal calf serum (FCS), development of Streptococcus mitis (S. mitis) growth inhibition zones on blood agar plates and mass spectrometry.
The microcapsules dissolved constantly over a time period of 32 days. The supernatants from the dissolving microcapsules that were used for the growing experiments of S. mitis on agar plates, demonstrated a time dependent development of inhibition zones of S. mitis growth from 1948 µm (24 hours) up to 633 µm (32 days) during the whole time of experiments that were still present after 32 days. Mass spectrometry of the supernatants revealed, that PHMB was detectable after every time point up to 32 days in concentration range from 4027 ng/µl (24 hours) to 930 ng/µl (32 days).
In conclusion, an anti-infective device with low toxicity, good tissue tolerance and high antibacterial efficacy which can be used as a long-term therapeutic agent with slow-release functionality was established.
Polihexanide; Periodontitis; Periodontal pockets; Slow release device; Antibacterial; Disinfection
Preview Article PDF
S. Groeger, T. Brandau and J. Meyle. Development of a slow-release device for pocket disinfection. GSC Biological and Pharmaceutical Sciences, 2022, 21(1), 188-196. Article DOI: https://doi.org/10.30574/gscbps.2022.21.1.0198