Development and validation of new RP-HPLC method for the estimation of ceftriaxone sodium in bulk and pharmaceutical dosage form

A simple, rapid, accurate and precise RP-HPLC method was developed and validated for the determination of ceftrioxone sodium in parentral dosage form. Chromatographic analysis of the drug was achieved on CYBER LAB HPLC comprising of LC- 100 P pump, a variable wavelength programmable LC-UV100 UV detector and SCL system controller. Flowrosil C18 column (250 mm x 4.6 mm, 5 μ) as stationary phase with mobile phase consisting of methanol: water : ortho-phosphoric acid in the ratio of (75 :24.5 :0.5 v/v ) . The method showed a good linear response in the concentration range of 10-50 μg/ml with correlation coefficient of 0.9990. The flow rate was maintained at 1.0 ml/min and detection was carried out at 240 nm. The retention time was 3.788 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, solution stability, selectivity and sensitivity. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the determination of ceftrioxone sodium in parentral formulation.