1 College of Medical Laboratory Sciences, Department of Hematology, Karary University, Khartoum Sudan.
2 College of Health Sciences, Department of Medical Laboratory Sciences, Gulf Medical University, AL JURF, Ajman, United Arab Emirates.
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 22(03), 218–224.
Article DOI: 10.30574/gscbps.2023.22.3.0091
Received on 01 February 2023; revised on 10 March 2023; accepted on 13 March 2023
Background: Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme regulating the intracellular folate metabolism which plays an important role in carcinogenesis through DNA methylation.
Objectives: The current study aimed to determine the association between MTHFR C677T polymorphisms and the risks of chronic myeloid leukemia (CML).
Material and methods: This are hospital based cross sectional case control study, a total of 170 Sudanese subjects were enrolled, and 75 patients with chronic myeloid leukemia and 75 age- and sex matched healthy volunteers as a control group. Genomic DNA was extracted by sodium chloride method and The SNPs genotypes were determined using polymerase chain reaction followed by restriction fragment length polymorphism method. (PCR- RFLP). Data of this study were collected using a structured interview questionnaire and analyzed by statistical package for social sciences (version 21).
Results: The frequency of the CC genotype was higher in the control group compared with patients (100%,96%), while of the CT genotype was higher in the case (4%) than in control (0.0%); the TT genotype was absent in both study groups, the frequencies T and C allele were 0.02 and 0.98 in CML patient group and 0.00, 0.100 respectively in the control group. There was no clinically significant association between age (P.value= 0.3), gender (P.value= 0.4) and genotype, also there was no statistically significant association when compare case with control genotype (p. value 0.08).
Conclusion: C677T MTHFR polymorphism is not associated with the risk of CML among the Sudanese population
Leukemia; Methylenetetrahydrofolate reductase; Polymorphism; Sudan
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Mubarak Mustafa Elkarsany, Nadia Madni Mohammed Ahmed, Abdelmohmoud Mokhtar Bashir and Abdegadir Alamin Altoum. Association of Methylenetetrahydrofolate Reductase (MTHFR) Gene C677T (rs1801133) polymorphism among Sudanese patients with chronic myelogenous leukemia. GSC Biological and Pharmaceutical Sciences, 2023, 22(3), 218-224. Article DOI: https://doi.org/10.30574/gscbps.2023.22.3.0091