Antimicrobial activities of silver nanoparticles synthesized from Helichrysum italicum (Roth) G. Don

In our study, the ability to produce silver nanoparticles (AgNP) by green synthesis method using Helichrysum italicum (Roth) G. Don. plant was investigated. The color of the extract changed from yellow to brown, proving the presence of AgNPs. Transmission electron microscope (TEM), scanning electron microscopy (SEM), and Energy-dispersive spectra (EDS), FT-IR and Uv-Vis analysis were used to characterized AgNPs. AgNPs ranged from 20 nm to 40 nm in size and were mostly spherical and cubic structures. The antimicrobial potential of AgNPs was tested against seven bacterial and one yeast cultures by disc diffusion and minimum inhibitory concentration (MIC) methods. AgNPs significantly inhibited all test cultures. The highest antimicrobial activities of plant extract and AgNP were found against Proteus vulgaris ATCC 13315.